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 Of AP1: AP1 (activator protein 1) Alerter l'administrateur Recommander à un ami Lien de l'article 

Is a transcription regulator, which combines the homologous sequences of 5 `-TGAGTCA-3`. When the gene promoter region of AP1 binding sites exist, these genes can be induced, such as phorbol ester-induced protein kinase C (2,7). The formation of c-Jun or Jun-related protein in the cell, AP1 together in a catamaran, or the formation of heterodimers of c-Jun or Jun-related protein and c-Fos or Fos-related antigens (Fras) catamaran. Fos protein can not form together in the body, and can not alone and AP1 binding site. c-Jun protein is a 40kDa monomeric protein and the formation through the leucine zipper together in catamaran. The main form of AP1 in HeLa cells, c-Jun protein with poly catamaran. To form a specific complex in gel mobility shift assay. As a gel mobility shift assay determination of AP1 activity, in addition to the basic solution group particularly, should be 0.01mg/ml poly (the dI: dC)? The dI: dC in), 100ug of BSA, 5mMDTT added to the binding buffer. With purified protein used 1-2ug of protein to detect the migration of the complexes.
Two. Of AP2: of AP2 is a transcription regulator, respectively, as the TPA-and cAMP-induced factor (10). It is a 52-kDa protein, the identification of homologous sequence 5 `-CCCCAGGC-3` or 5 `-GCCNNGGC-3` (3). This factor is particularly sensitive to retinoic acid, may play an important role in morphogenesis. To form a specific complex in HeLa nuclear extract and AP2 homologous DNA probes. Gel mobility shift assay using purified protein, the application of 20-50ng of protein. Of CREB: CREB is a transcription regulator in a 37-kDa on the cAMP response, identify the 5 `-T (G / T) ACGTCA-3` DNA homologous sequences (6,11). It contains a leucine zipper structure formed together in the body, the basic structure of the domain and c-JunDNA binding domain homologous. When HeLa nuclear extract, and CREB homologous sequence to form a complex.

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  Aucun commentaire | Ecrire un nouveau commentaire Posté le 29-05-2012 à 07h59

 Membrane proteins dissolved Alerter l'administrateur Recommander à un ami Lien de l'article 

The dissolution is the most critical steps in the membrane protein preparation process. Through the process of dissolution, detergent membrane proteins from which the natural environment (lipid membranes) to extract water-soluble environment. Detergents break the lipid bilayer and membrane proteins and lipids wrapped into the detergent micelles. The hydrophobic tail of the hydrophobic surface of membrane proteins and lipid molecules are buried in the detergent micelles internal, while the hydrophilic part in contact with the aqueous solution environment (Fig1.1). The detergent can effectively break the lipids - the interaction between the membrane protein, protein - protein, it can be used for protein separation. In the presence of the detergent, known for water-soluble protein purification technology can be used for the purification of membrane proteins. Successful dissolution steps can ensure a stable protein - detergent complexes (or protein - lipid - detergent complexes), and the protein also maintained its activity when the natural conformation, the yield should be higher . Related Articles: Basic fibroblast growth factor http://www.creativebiomart.net/symbolsearch_FGF2.htm

  Aucun commentaire | Ecrire un nouveau commentaire Posté le 17-06-2012 à 06h38

 Toxic effects of antibiotics Alerter l'administrateur Recommander à un ami Lien de l'article 

Animal use of antibiotics, the body may be some residual antibiotics. The long-term intake of aminoglycoside antibiotics for Excessive animal products can lead to nephrotoxicity and ototoxicity, renal toxicity damage to renal tubular epithelial cells proteinuria, hematuria, severe renal dysfunction; ototoxicity mainly vestibular dysfunction and cochlear nerve damage. Long-term intake containing chloramphenicol residues in animal products can cause aplastic anemia. related articles:Thromboplastin

  Aucun commentaire | Ecrire un nouveau commentaire Posté le 05-07-2012 à 05h28

 Resistance Alerter l'administrateur Recommander à un ami Lien de l'article 

Antibiotics residues in animal products may make the animal pathogens to develop resistance, bacterial resistance gene by transformation, transduction, conjugation, such as translocations ask spread of the bacteria, resistance genes may also be spread through the food chain and other ways, so that resistance genes in the population of bacterial fauna, bacteria and ecosystems, bacteria with each other to pass, which led to the increase in resistance of the pathogens and cause human and animal infectious diseases, treatment failure, harm to human health, such as The average resistance rates of Staphylococcus aureus to 23 kinds of commonly used antibiotics in 2003-2006 were 56%, 66%, 71%, 67% [1]. related articles:Thromboplastin

  Aucun commentaire | Ecrire un nouveau commentaire Posté le 05-07-2012 à 05h29

 Destruction of the micro-ecological balance of the body Alerter l'administrateur Recommander à un ami Lien de l'article 

The intestinal tract of livestock and poultry, both harmful and pathogenic microorganisms, the growth of beneficial micro-organisms, such as Lactobacillus and Bifidobacterium. Under normal circumstances the body of microorganisms mutual restraint in balance. The abuse of antibiotics inhibit or kill pathogenic microorganisms at the same time state equilibrium, resulting in animal morbidity or mortality. related articles:Thromboplastin

  Aucun commentaire | Ecrire un nouveau commentaire Posté le 05-07-2012 à 05h29

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  Blog créé le 29-05-2012 à 07h53 | Mis à jour le 30-12-2012 à 21h17 | Note : Pas de note